Transcriptome Heat Map

Immune responses in bats

North American bats affected by white-nose syndrome

White-nose syndrome (WNS) has had catastrophic effects on bat populations in North America, with millions of bats from several species dying. Students in the Field and Reeder labs are studying how the cold-loving fungus that causes WNS, Pseudogymnoascus destructans (Pd), affects bats while they hibernate. By learning which specific types of immune responses Myotis lucifugus (little brown bats) can mount and the consequences of these immune responses, we hope to better understand bat immune physiology and the role of immune responses in WNS. With support from the US Fish and Wildlife Service, we hope that these studies will lead to the development of treatment strategies that can mitigate this potential extinction crisis. We are using a combination of approaches to determine how different bat species respond to Pd infection. Students in my lab are using whole-transcriptome approaches with next-generation sequencing (RNA-Seq). We also use quantitative PCR to measure the expression of specific genes and functional assays of memory immune responses. Because some species of bats are resistant to WNS (particularly European bats), we hope to identify protective responses that could then be augmented in susceptible species to promote WNS survival. We are also studying remnant bat populations in Pennsylvania to determine how some small groups of little brown bats have adapted to living with WNS. Read more about the Field-Reeder lab collaboration here.

WNS on a batHibernation and immune responses

Bats are unique among mammals due to the metabolic demands of flight. In North America and Europe they are highly seasonal, using migration and/or hibernation to survive temperate winters. Bats are ecologically important animals because of their role as nocturnal predators of insects. For these reasons, I am interested in understanding the immune responses of bats and how their immune physiology compares to other hibernating and non-hibernating mammals. Together with the Reeder lab, my students are studying how hibernation affects immune function and gene expression.

Zoonotic diseases in bats

Fruit bats in Africa are reservoirs for many viral diseases that can spill over to humans including Ebola, Marburg, Nipah, and Hendra viruses. Students in the Field and Reeder labs are using next-generation RNA sequencing (RNA-Seq) to understand immune physiology and other metabolic processes within bats that contribute to disease spread. With support from the National Institutes of Health, we will determine how differences in gene expression influence immune competence as a function of time of year, sex, age, and reproductive status. We will use this information to better understand how bats serve as disease vectors, to mitigate zoonotic disease outbreaks, and to prevent potential spillover events by predicting conditions that favor disease transmission.

Undergraduate Research Projects

Bucknell students interested in conducting research with Prof. Field should contact him by e-mail (ken.field@bucknell.edu ) to arrange an appointment. Opportunities are available for summer research or for Biol 399 research. Most research projects span at least two semesters and the summer so interested students should become involved in their sophomore or junior year. Students are expected to complete Immunology (Biol 348) while enrolled in research.

Current Students

Publications

Research Grants

June 17th, 2017

Three new papers published!

First, our study of the immune response in little brown bats infected with P. destructans came out in Proceedings of the Royal Society B. Second, our paper describing the differences in how WNS affects susceptible little brown bats and resistant big brown bats was just published in Journal of Comparative Physiology B. Third, our paper that shows the changes in gene expression when P. destructans is infecting bats compared to when it grows in culture is in press at Virulence. Exciting times for the Bucknell bat lab!

Continue reading Three new papers published! »

April 13th, 2016

Frontiers in Zoology paper in the news!

Our first paper studying the remnant bat populations that are persisting in the face of white-nose syndrome has been covered all over the news, thanks to an excellent article written by Michael Hill of the Associated Press. Here are a few of the links (of about 1,080 results as of Apr 13, according to Google): CBS News The Enterprise News in Brief

Continue reading Frontiers in Zoology paper in the news! »

Close

Places I've Been

The following links are virtual breadcrumbs marking the 12 most recent pages you have visited in Bucknell.edu. If you want to remember a specific page forever click the pin in the top right corner and we will be sure not to replace it. Close this message.